Rabbit Anti-Human NQO1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P15559 |
| Gene Id | 1728 |
| Gene Symbols | NQO1 |
| Protein Name / Synonyms | NQO1 (Center) |
| Clonality | Polyclonal |
| Preparation | This NQO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the Central region of human NQO1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NQO1 is a member of the NAD(P)H dehydrogenase(quinone) family and a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).
Specificity
Other Names
NQO1; DIA4; NMOR1; NAD(P)H dehydrogenase [quinone] 1; Azoreductase; DT-diaphorase; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1
NCBI Accession #
NP_000894.1;NP_001020604.1;NP_001020605.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
30868
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of NQO1 antibody (Center) (Cat# 102-17297) in NCI-H460 cell line lysates (35ug/lane). NQO1 (arrow) was detected using the purified Pab. |
 | NQO1 antibody (Center) (Cat# 102-17297) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of NQO1 Antibody (Center)(Cat# 102-17297) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.