NR0B1 antibody, 0.4 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 190 |
| Protein Name / Synonyms | NR0B1 |
| Concentration (lot specific) | 0.4 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AHC antibody, AHCH antibody, AHX antibody, DAX-1 antibody, DAX1 antibody, DSS antibody, GTD antibody, HHG antibody, NROB1 antibody, NR0B1 antibody, nuclear receptor DAX1 antibody, nuclear receptor subfamily 0 group B member 1 antibody, nuclear hormone receptor antibody, nuclear receptor DAX-1 antibody, DSS-AHC critical region on the X chromosome protein 1 antibody, nuclear receptor subfamily 0, group B, member 1 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
52 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 96 and 406 of NR0B1 (Uniprot ID# P51843)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NR0B1(107-10516) antibody at 1:100 dilution.
NRB1 antibody immunoprecipitates NRB1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of NRB1 protein by 2 µg of NRB1 antibody (107-10516) 7.5% SDS-PAGE The immunoprecipitated NRB1 protein was detected by NRB1 antibody (107-10516) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using DAX-1(107-10516) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: HeLa S3
7.5% SDS PAGE
107-10516 diluted at 1:500
A: HeLa S3
7.5% SDS PAGE
107-10516 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.