Synonyms
FLJ17090 antibody, SHP antibody, SHP1 antibody, NR0B2 antibody, orphan nuclear receptor SHP antibody, small heterodimer partner antibody, nuclear receptor SHP antibody, nuclear receptor subfamily 0 group B member 2 antibody, "nuclear receptor subfamily 0, group B, member 2 antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
28 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 64 and 257 of NR0B2 (Uniprot ID# Q15466)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using NR0B2(107-11094) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using NR0B2(107-11094) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.