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Anti-NR2C2 (C-term) Antibody
Catalog #: 102-14242
Rabbit Anti-Human NR2C2 (C-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P49116 |
| Gene Id | 7182 |
| Gene Symbols | NR2C2 |
| Protein Name / Synonyms | NR2C2 (C-term) |
| Clonality | Polyclonal |
| Preparation | This NR2C2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-466 amino acids from the C-terminal region of human NR2C2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Members of the nuclear hormone receptor family, such as NR2C2, act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain, a central DNA-binding domain with 2 zinc fingers, and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al., 1996 [PubMed 8661150]).
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
NR2C2; TAK1; TR4; Nuclear receptor subfamily 2 group C member 2; Orphan nuclear receptor TAK1; Orphan nuclear receptor TR4; Testicular receptor 4
NCBI Accession #
NP_003289.2
Other Accession #
P55094; P49117; NP_003289.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
65414
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | NR2C2 Antibody (C-term) (Cat# 102-14242) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NR2C2 antibody detected the NR2C2 protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with NR2C2 Antibody (C-term)(Cat# 102-14242).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR2C2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NR2C2 immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.