Anti-NR3C1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P04150
Gene Id	2908
Gene Symbols	NR3C1
Protein Name / Synonyms	NR3C1 (Center)
Clonality	Polyclonal
Preparation	This NR3C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-262 amino acids from the Central region of human NR3C1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NR3C1 is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined.

Specificity

Predicted Reactivity

Rabbit

Other Names

NR3C1; GRL; Glucocorticoid receptor; Nuclear receptor subfamily 3 group C member 1

NCBI Accession #

NP_000167.1;NP_001018084.1;NP_001018085.1;NP_001018086.1;NP_001018087.1;NP_001018661.1;NP_001019265.1;NP_001191187.1;NP_001191188.1;NP_001191189.1;NP_001191190.1;NP_001191191.1;NP_001191192.1;NP_00119

Other Accession #

P59667; NP_000167.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

85659

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	NR3C1 Antibody (Center) (Cat# 102-16504) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the NR3C1 antibody detected NR3C1 protein (arrow).
	NR3C1 Antibody (Center) (Cat# 102-16504)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NR3C1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Fluorescent confocal image of Hela cell stained with NR3C1 Antibody (Center)(Cat# 102-16504).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NR3C1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NR3C1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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