Anti-NRG1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q02297
Gene Id	3084
Gene Symbols	NRG1
Protein Name / Synonyms	NRG1 (Center)
Clonality	Polyclonal
Preparation	This NRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-229 amino acids from the Central region of human NRG1.
Concentration (lot specific)	1.790 mg/ml
Recommended Applications	IHC-P, FC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.

Specificity

Other Names

NRG1; GGF; HGL; HRGA; NDF; SMDF; Pro-neuregulin-1, membrane-bound isoform; Neuregulin-1; Acetylcholine receptor-inducing activity; Breast cancer cell differentiation factor p45; Glial growth factor; Heregulin; Neu differentiation factor; Sensory and motor neuron-derived factor

NCBI Accession #

NP_001153467.1;NP_001153471.1;NP_001153473.1;NP_001153477.1;NP_001153480.1;NP_039250.2;NP_039251.2;NP_039252.2;NP_039253.1;NP_039254.1;NP_039256.2;NP_039258.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

70392

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:50-100

	Western blot analysis of anti-NRG1 Antibody (Center) (Cat# 102-16685) in SK-BR-3 cell line lysates (35ug/lane). NRG1 (arrow) was detected using the purified Pab.
	Flow cytometric analysis of NCI-H460 cells using NRG1 Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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