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Anti-NSMCE1 Antibody
Catalog #: 107-10668
NSMCE1 antibody [N1C3], 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 197370 |
| Protein Name / Synonyms | NSMCE1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
NSE1 antibody, NSMCE1 antibody, non-structural maintenance of chromosomes element 1 homolog antibody, non-SMC element 1 homolog (S. cerevisiae) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
31 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 266 of NSMCE1 (Uniprot ID# Q8WV22)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using NSMCE1(107-10668) antibody at 1:500 dilution.
Sample(30 ug whole cell lysate)
A:Raji
12% SDS PAGE
107-10668 diluted at 1:2000
A:Raji
12% SDS PAGE
107-10668 diluted at 1:2000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.