Anti-NT5C2 (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	22978
Protein Name / Synonyms	NT5C2, Internal
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

GMP antibody, NT5B antibody, PNT5 antibody, cN-II antibody, NT5C2 antibody, cytosolic 5'-nucleotidase II antibody, "5'-nucleotidase (purine), cytosolic type B antibody", IMP-specific 5'-NT antibody, purine 5' nucleotidase antibody, cytosolic purine 5'-nucleotidase antibody, "5'-nucleotidase, cytosolic II antibody"

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

65 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 108 and 362 of NT5C2 (Uniprot ID# P49902)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
107-10554 diluted at 1:500

Immunohistochemical analysis of paraffin-embedded OVCA, using NT5C2(107-10554) antibody at 1:100 dilution.

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3
7.5% SDS PAGE
107-10554 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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