RayBiotech

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ISO 13485:2016 cGMP

Anti-NULP1 Antibody

Catalog #: 107-10318

NULP1 antibody [C1C3], 1 mg/ml (100 ul)

Product Description

Specifications

Size100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Gene Id
22980
Protein Name / Synonyms
NULP1
Concentration (lot specific)
1 mg/ml
Recommended ApplicationsICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping TypeBlue ice
Storage-20°C

Description

Synonyms

Hulp1 antibody, KIAA1049 antibody, NULP1 antibody, PRO2620 antibody, hKIAA1049 antibody, TCF25 antibody, TCF-25 antibody, nuclear localized protein 1 antibody, transcription factor 25 antibody, transcription factor 25 (basic helix-loop-helix) antibody

Specificity

Species Reactivity
Human, Mouse

Format

Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
77 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 427 and 676 of NULP1 (Uniprot ID# Q9BQ70)

Applications

Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-10318 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NULP1(107-10318) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, using NULP1(107-10318) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
107-10318 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.