Anti-NuMA (N-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	4926
Protein Name / Synonyms	NuMA, N-term
Concentration (lot specific)	0.42 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

NUMA antibody, NUMA1 antibody, nuclear mitotic apparatus protein 1 antibody, centrophilin stabilizes mitotic spindle in mitotic cells antibody, SP-H antigen antibody, structural nuclear protein antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

238 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 339 of NuMA (Uniprot ID# Q14980)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:2000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using NuMA(107-11070) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

NuMA antibody [N1], N-term detects NUMA1 protein by Western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg A375 whole cell lysate/extract
5 % SDS-PAGE
NuMA antibody [N1], N-term (107-11070) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using NuMA (107-11070) antibody at 1:100 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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