O-GlcNAc transferase antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 8473 |
| Protein Name / Synonyms | O-GlcNAc transferase |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Post-Translational Modifications, Glycosylation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
FLJ23071 antibody, HRNT1 antibody, MGC22921 antibody, O-GLCNAC antibody, OGT antibody, O-GlcNAc transferase p110 subunit antibody, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase antibody, O-GlcNAc transferase subunit p110 antibody, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody, O-linked N-acetylglucosamine transferase 110 kDa subunit antibody, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
117 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 223 and 502 of O-GlcNAc transferase (Uniprot ID# O15294)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using O-GlcNAc transferase(107-10883) antibody at 1:200 dilution.
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg A431 whole cell extract
C. 30 µg HeLa whole cell extract
D. 30 µg HepG2 whole cell extract
7.5 % SDS-PAGE
O-GlcNAc transferase antibody (107-10883) dilution: 1:1000
A. 30 µg 293T whole cell extract
B. 30 µg A431 whole cell extract
C. 30 µg HeLa whole cell extract
D. 30 µg HepG2 whole cell extract
7.5 % SDS-PAGE
O-GlcNAc transferase antibody (107-10883) dilution: 1:1000
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.
A. 50 µg mouse brain extract
5 % SDS-PAGE
O-GlcNAc transferase antibody (107-10883) dilution: 1:1000
A. 50 µg mouse brain extract
5 % SDS-PAGE
O-GlcNAc transferase antibody (107-10883) dilution: 1:1000
O-GlcNAc transferase antibody detects O-GlcNAc transferase protein by western blot analysis.
A. 30 µg PC-12 whole cell extract
5 % SDS-PAGE
O-GlcNAc transferase antibody (107-10883) dilution: 1:1000
A. 30 µg PC-12 whole cell extract
5 % SDS-PAGE
O-GlcNAc transferase antibody (107-10883) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using O-GlcNAc transferase (107-10883) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.