Anti-O-GlcNAc transferase (N-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	8473
Protein Name / Synonyms	O-GlcNAc transferase, N-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Post-Translational Modifications, Glycosylation
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

FLJ23071 antibody, HRNT1 antibody, MGC22921 antibody, O-GLCNAC antibody, OGT antibody, O-GlcNAc transferase p110 subunit antibody, uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase antibody, O-GlcNAc transferase subunit p110 antibody, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit antibody, O-linked N-acetylglucosamine transferase 110 kDa subunit antibody, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

117 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 280 of O-GlcNAc transferase (Uniprot ID# O15294)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

O-GlcNAc transferase antibody [N1N2], N-term detects OGT protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
O-GlcNAc transferase antibody [N1N2], N-term (107-10902) dilution: 1:500.

O-GlcNAc transferase antibody [N1N2], N-term detects O-GlcNAc transferase protein at cytosol on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
O-GlcNAc transferase antibody [N1N2], N-term (107-10902) dilution:

Sample (50 ug of whole cell lysate)
A: Mouse brain
5% SDS PAGE
107-10902 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
107-10902 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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