3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Obestatin Antibody, Biotinylated
Catalog #: RB-08-0026B-50
Rabbit anti-Obestatin Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | NM_016362 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human Obestatin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CFNAPFDVGIKLSGVQTQQHSQAL |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1-2 µg/ml), Western Blotting (recommended work dilution= 1:1000-2000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Ghrelin and obestatin are a single gene products and are a multiple functional peptides that regulates energy homeostasis, and food intake. Both are two different peptides generated from proteolytic cleavage of the same precursor, preproghrelin by posttranslational processing. Human obestatin consists of 23 amino acid peptide with a flanking conserved glycine residue at the C-terminus, in which has very high homology with ones derived from other species.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-human Obestatin antibody detects specifically both synthetic peptide and cellular protein from 3T3-L1 cell line. Cross reactivity with mouse and rat are expected from sequence similarity.
References
- Zhang JV, Ren PG, Avsian-Kretchmer O, et al. (2005). "Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake". Science 310 (5750): 9969. PMID 16284174.
Expiration:
12 months from the date of shipment when stored properly.