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Anti-Obestatin Antibody
Catalog #: RB-08-0026
Rabbit Anti-Human Obestatin Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NM_016362 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human Obestatin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CFNAPFDVGIKLSGVQTQQHSQAL |
| Recommended Applications | ELISA (recommended work dilution= 1-2 µg/ml), Western Blotting (recommended work dilution= 1:1000-2000) |
| Reconstitution | The purified antibody is supplied lyophilized originally containing PBS without any preservative stabilizers. The lyophilized antibody is stable for at least a year from the date of receipt when stored frozen at -20°C to -70°C. It is suggested that the antibody is reconstituted with sterile water and can be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for months at 4°C. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Ghrelin and obestatin are a single gene products and are a multiple functional peptides that regulates energy homeostasis, and food intake. Both are two different peptides generated from proteolytic cleavage of the same precursor, preproghrelin by posttranslational processing. Human obestatin consists of 23 amino acid peptide with a flanking conserved glycine residue at the C-terminus, in which has very high homology with ones derived from other species.
Specificity
The rabbit anti-human Obestatin antibody detects specifically both synthetic peptide and cellular protein from 3T3-L1 cell line. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.
References
- Zhang JV, Ren PG, Avsian-Kretchmer O, et al. (2005). "Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin's effects on food intake". Science 310 (5750): 9969. PMID 16284174.
Expiration:
12 months from the date of shipment when stored properly.