Rabbit Anti-Human OCT4 (OCT3) (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q01860 |
| Gene Id | 5460 |
| Gene Symbols | POU5F1 |
| Protein Name / Synonyms | OCT4 (OCT3) (N-term) |
| Clonality | Polyclonal |
| Preparation | This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human OCT4 (OCT3). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.
Specificity
Other Names
POU5F1; OCT3; OCT4; OTF3; POU domain, class 5, transcription factor 1; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3
NCBI Accession #
NP_002692.2
Other Accession #
NP_002692
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
38571
Recommended Dilutions
WB: 1:1000; IF: 1:50-100
 | The anti-OCT4 (OCT3) (N-term) Pab (Cat# 102-14813) is used in Western blot to detect OCT4 (OCT3) in A375 cell lysate. |
 | Immunofluorescence analysis of OCT3(OCT4) antibody (N-term) (Cat# 102-14813) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.