Anti-OGT Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O15294
Gene Id	8473
Gene Symbols	OGT
Protein Name / Synonyms	OGT, HINCUT-1, HRNT1, O-GLCNAC, MRX106, OGT1, O-linked N-acetylglucosamine (GlcNAc) transferase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

74kDa/103kDa/115kDa/116kDa

Observed MW

118kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 857-1036 of human OGT (NP_858059.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using OGT antibody (144-01990) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - OGT antibody (144-01990)

Immunofluorescence analysis of U2OS cells using OGT antibody (144-01990). Blue: DAPI for nuclear staining.
Immunofluorescence - OGT antibody (144-01990)

Expiration:

12 months from the date of shipment when stored properly.

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