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Anti-Orexin A Antibody
Catalog #: RB-13-0009
Rabbit Anti-Human Orexin A Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | O43612 |
| Gene Id | 3060 |
| Gene Symbols | HCRT OX PPORX PPOX |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human Orexin A. This antibody was obtained from a rabbit immunized with the immunogen. The IgG fraction of immunized serum was purified by affinity chromatographies. |
| Immunogen Sequence | EPLPDCCRQKTCSCRLYELLHGAGNHAAGILTL |
| Recommended Applications | ELISA (recommended work dilution= 1:2, 000-5, 000(at least)) |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Orexin A is active biological orexigenic peptide composed of 33 amino acids. It and Orexin B were derived from the same precursor peptide (131 amino acids) by proteolytic processing and are highly specifically localized in neurons located in the lateral hypothalamic area, a region classically implicated in feeding behavior. Both neuropeptides have no significant structural similarities to known families of regulatory peptides. They plays a central role in the integrated control of feeding and energy homeostasis.
Specificity
The antibody is able to bind to immunized peptide, but not other unrelated peptides in ELISA. It also specifically detects cellular protein derived from selected cell lines. This antibody shows no crossreactivity with other unrelated protein.
Storage
Always prepare single-use aliquots planning use while receiving package. A small amount of antibody can be kept at 4°C for daily use. For a long storage, recommend to keep them at -20°C after adding an equal volume of glycerol in PBS. It will not lose activity over a year. Minimize freezing and thawing when use.
References
- Sakurai T., Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell 92:573-585(1998)
- Sakurai T., Structure and function of human prepro-orexin gene." J. Biol. Chem. 274:17771- 17776(1999)
Expiration:
12 months from the date of shipment when stored properly.