Anti-Osteocalcin (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P02818
Gene Id	632
Gene Symbols	BGLAP
Protein Name / Synonyms	Osteocalcin (N-term)
Clonality	Polyclonal
Preparation	This Osteocalcin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human Osteocalcin.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Prior to the formation of calcified bone, noncollagenous proteins form in the extracellular bone matrix. Gamma-carboxyglutamic acid residues are formed by vitamin K, vitamin-D regulated calcium binding proteins containing residues of Gla. These residues are essential for the binding of calcium and constitue 1-2% of total bone protein. Osteocalcin itself binds strongly to apatite and calcium. Production of osteocalcin is expressed late in normal bone development and is characteristic of mature osteoblasts. Regular osteocalcin production has been shown to be linked to the p53 tumor suppressor gene. The p53 gene undergoes rearrangement in a high percentage of osteosarcomas, resulting in loss of its expression. The loss of p53 regulation inhibits further osteocalcin production. The absence of end-point differentiation in bone due to p53 rearrangements and lack of osteocalcin production may contribute to the maintenance of the tumorigenic phenotype in osteosarcomas.

Specificity

Other Names

BGLAP; Osteocalcin; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein

NCBI Accession #

NP_954642.1

Other Accession #

NP_954642

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

10963

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of Osteocalcin (arrow) using rabbit polyclonal Osteocalcin Antibody (N-term) (Cat# 102-14512).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BGLAP gene (Lane 2) (Origene Technologies).
	Osteocalcin Antibody (N-term) (Cat# 102-14512) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the Osteocalcin antibody detected the Osteocalcin protein (arrow).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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