Synonyms
ARC21 antibody, p21-Arc antibody, ARPC3 antibody, arp2/3 complex 21 kDa subunit antibody, ARP2/3 protein complex subunit p21 antibody, actin-related protein 2/3 complex subunit 3 antibody, "actin related protein 2/3 complex, subunit 3, 21kDa antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
21 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 178 of p21-ARC (Uniprot ID# O15145)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded endo mitral OVCA, using ARPC3(107-10306) antibody at 1:100 dilution.

Sample(30 µg of whole cell lysate)
A:Raji
12% SDS PAGE
107-10306 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.