Anti-P4HB (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P07237
Gene Id	5034
Gene Symbols	P4HB
Protein Name / Synonyms	P4HB (C-term)
Clonality	Polyclonal
Preparation	This P4HB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-464 amino acids from the C-terminal region of human P4HB.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

P4HB is a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Hamster

Other Names

P4HB; ERBA2L; PDI; PDIA1; PO4DB; Protein disulfide-isomerase; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55

NCBI Accession #

NP_000909.2

Other Accession #

P04785; P09103; Q8R4U2; P05307

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

57116

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of P4HB Antibody (C-term) (Cat# 102-15220) in CEM,HepG2,A2058 cell line lysates (35ug/lane). P4HB (arrow) was detected using the purified Pab.(2ug/ml)
	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with P4HB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of K562 cells using P4HB Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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