Anti-p53 (Sumoylation Site Specific) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04637
Gene Id	7157
Gene Symbols	TP53
Protein Name / Synonyms	p53 (Sumoylation Site Specific)
Clonality	Polyclonal
Preparation	This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from human p53.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Apoptosis, Cancer, Post-Translational Modifications, p53 Signaling, Autophagy, DNA Damage
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.

Specificity

Other Names

Cellular tumor antigen p53; Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; TP53

NCBI Accession #

NP_000537.3;NP_001119584.1;NP_001119585.1;NP_001119586.1;NP_001119587.1;NP_001119588.1;NP_001119589.1;NP_001119590.1;NP_001263624.1;NP_001263625.1;NP_001263626.1;NP_001263627.1;NP_001263628.1;NP_00126

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

43653

Recommended Dilutions

WB: 1:1000

TP53 Antibody (C-term) (Cat# 102-15040) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TP53 antibody detected the TP53 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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