Anti-p63 (internal) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	8626
Protein Name / Synonyms	p63, Internal
Concentration (lot specific)	0.34 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

AIS antibody, B(p51A) antibody, B(p51B) antibody, EEC3 antibody, KET antibody, LMS antibody, NBP antibody, OFC8 antibody, RHS antibody, SHFM4 antibody, TP53CP antibody, TP53L antibody, TP73L antibody, p40 antibody, p51 antibody, p53CP antibody, p63 antibody, p73H antibody, p73L antibody, TP63 antibody, keratinocyte transcription factor KET antibody, CUSP antibody, amplified in squamous cell carcinoma antibody, tumor protein p53-competing protein antibody, tumor protein p63 deltaN isoform delta antibody, chronic ulcerative stomatitis protein antibody, tumor protein 63 antibody, transformation-related protein 63 antibody, tumor protein p63 antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

77 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 125 and 415 of p63 (Uniprot ID# Q9H3D4)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using p63(107-10336) antibody at 1:100 dilution.

p63 antibody detects p63 protein by western blot analysis.
A. 30 µg A431 whole cell lysate/extract
7.5 % SDS-PAGE
p63 antibody (107-10336) dilution: 1:500

p63 antibody [N2C1], Internal detects TP63 protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
7.5 % SDS-PAGE
p63 antibody [N2C1], Internal (107-10336) dilution: 1:500

p63 antibody [N2C1], Internal detects TP63 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
7.5 % SDS-PAGE
p63 antibody [N2C1], Internal (107-10336) dilution: 1:500

Immunofluorescence analysis of paraformaldehyde-fixed A431, using p63 (107-10336) antibody at 1:200 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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