Synonyms
PABP3 antibody, PABPL3 antibody, tPABP antibody, PABPC3 antibody, poly(A)-binding protein 3 antibody, testis PABP antibody, PABP-3 antibody, poly(A)-binding protein-like 3 antibody, polyadenylate-binding protein 3 antibody, testis-specific poly(A)-binding protein (PABP) antibody, testis-specific poly(A)-binding protein 3 antibody, "poly(A) binding protein, cytoplasmic 3 antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
70 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 107 and 323 of PABPC3 (Uniprot ID# Q9H361)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using PABPC3(107-11130) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: JurKat
7.5% SDS PAGE
107-11130 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.