Anti-PACSIN2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9UNF0
Gene Id	11252
Gene Symbols	PACSIN2
Protein Name / Synonyms	PACSIN2 (C-term)
Clonality	Polyclonal
Preparation	This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from the C-terminal region of human PACSIN2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PACSIN may play a role in vesicle formation and transport. This protein homo- and hetero-aggregates with other PACSINs. It also binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). The protein exhibits a cvesicle-like cytoplasmic distribution and is ubiquitously expressed. PACSIN is phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). The protein contains 1 FCH domain and 1 SH3 domain.

Specificity

Other Names

PACSIN2; Protein kinase C and casein kinase substrate in neurons protein 2

NCBI Accession #

NP_001171899.1;NP_001171900.1;NP_009160.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55739

Recommended Dilutions

IF: 1:100; WB: 1:1000; IHC: 1:50-100

	Immunofluorescent analysis of Hela cells, using PACSIN2 Antibody (C-term) (Cat# 102-17794). 102-17794 was diluted at 1:100 dilution. Alexa Fluor^® 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).Cytoplasmic actin was counterstained with Dylight Fluor^® 554 (red) conjugated Phalloidin (red).
	PACSIN2 Antibody (S357) (Cat# 102-17794) western blot analysis in Daudi cell line and mouse brain lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).
	PACSIN2 Antibody (S357) (Cat# 102-17794) western blot analysis in Daudi,HL-60 cell line and mouse brain,rat heart lysates (35ug/lane).This demonstrates the PACSIN2 antibody detected the PACSIN2 protein (arrow).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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