Anti-PAK1 (T423) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13177\|Q61036\|Q13153\|O75914\|Q8AXB4\|Q62829\|Q64303\|Q29502\|Q8CIN4\|P35465\|O88643\|Q08E52
Gene Id	5058
Gene Symbols	PAK1
Protein Name / Synonyms	Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK, PAK1
Clonality	Polyclonal
Clone	RB11382
Isotype	Kappa, Rabbit
Recommended Applications	Western Blotting, IF, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.

Other Information

NCBI Accession #

NP_001122092.1;NP_002567.3

Antigen Type

Synthetic Peptide

Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Calculated Molecular Weight (Da)

60647

Antigen Source

Human

Images

WB (1:1000)

Western blot analysis of PAK1 (arrow) using rabbit polyclonal PAK1 Antibody (T423) (Cat.#102-27438).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2) (Origene Technologies).

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products