Phospho-PAK2-S20 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13177 |
| Gene Id | 5062 |
| Gene Symbols | PAK2 |
| Protein Name / Synonyms | PAK2, PAK65, PAKgamma, p21 (RAC1) activated kinase 2 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
58kDa
Observed MW
58kDa
Immunogen
A synthetic phosphorylated peptide around S20 of human PAK2 (NP_002568.2).
Modification
Phospho S20
Storage
-20°C
Images
Western blot analysis of extracts of HeLa and NIH/3T3 cells, using Phospho-PAK2-S20 antibody (144-61527) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.