Anti-PAK5 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9P286
Gene Id	57144
Gene Symbols	PAK7
Protein Name / Synonyms	PAK5
Clonality	Polyclonal
Preparation	This PAK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-198 amino acids from human PAK5.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PAK5 is a member of the PAK family of Ser/Thr protein kinases. PAK family members are known to be effectors of Rac/Cdc42 GTPases, which have been implicated in the regulation of cytoskeletal dynamics, proliferation, and cell survival signaling. This kinase contains a CDC42/Rac1 interactive binding (CRIB) motif, and has been shown to bind CDC42 in the presence of GTP. This kinase is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. This kinase is associated with microtubule networks and induces microtubule stabilization. The subcellular localization of this kinase is tightly regulated during cell cycle progression.

Specificity

Other Names

PAK7; KIAA1264; PAK5; Serine/threonine-protein kinase PAK 7; p21-activated kinase 5; p21-activated kinase 7

NCBI Accession #

NP_065074.1;NP_817127.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

80745

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

Western blot analysis of anti-PAK5 Pab (Cat# 102-17705) in lysates from transiently transfected COS7 cells. Lane 1: negative control, Lane 2: PAK1-expressing cells, Lane 3: PAK2-expressing cells, Lane 4: PAK4-expressing cells, Lane 5: PAK5-expressing cells, and Lane 6: PAK6-expressing cells. PAK5 (arrow) was detected using purified Pab. Data is kindly provided by Drs. Z.M. Jaffer and J. Chernoff from the Fox Chase Cancer Center (Philadelphia, PA).

Formalin-fixed and paraffin-embedded human brain tissue reacted with PAK5 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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