Anti-Pan SUMO Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	P55854
Gene Id	6612
Gene Symbols	SUMO3
Protein Name / Synonyms	Pan SUMO
Clonality	Polyclonal
Preparation	This Pan SUMO antibody recognizes all 3 SUMO isoforms, including human SUMO1, SUMO2 and SUMO3. This antibody is generated from rabbits immunized with a recombinant protein encoding full length human SUMO3.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Post-Translational Modifications
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Covalent modification of target lysines by SUMO (small ubiquitin-like modifier) modulates processes such as protein localization, transcription, nuclear transport, mitosis, DNA replication and repair, signal transduction, and viral reproduction. SUMO does not seem to be involved in protein degradation and may in fact function as an antagonist of ubiquitin in the degradation process. The SUMO family consists of SUMO1 and closely related homologs SUMO2, SUMO3, and SUMO4. Sumoylation has been shown to regulate a wide range of proteins, including MDM2, PIAS, PML, RanGAP1, RanBP2, p53, p73, HIPK2, TEL, c-Jun, Fas, Daxx, TNFRI, Topo-I, Topo-II, PARK2, WRN, Sp100, IkB-alpha, Androgen receptor (AR), GLUT1/4, CaMK, DNMT3B, TDG, HIF1A, CHD3, EXOSC9, RAD51, and viral targets such as CMV-IE1/2, EBV-BZLF1, and HPV/BPV-E1.

Specificity

Other Names

SUMO3; SMT3B; SMT3H1; Small ubiquitin-related modifier 3; SMT3 homolog 1; SUMO-2; Ubiquitin-like protein SMT3B

NCBI Accession #

NP_008867.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

11637

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:50-100

	SUMO3 Antibody (Cat# 102-11786) western blot analysis in 293 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the SUMO3 antibody detected the SUMO3 protein (arrow).
	Fluorescent confocal image of Hela cell stained with Pan SUMO Antibody(Cat# 102-11786).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with Pan SUMO primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).Pan SUMO immunoreactivity is localized to Nucleus significantly.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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