PANK1 antibody [N3C3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 53354 |
| Protein Name / Synonyms | PANK1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
MGC24596 antibody, PANK antibody, PANK1a antibody, PANK1b antibody, PANK1 antibody, pantothenic acid kinase 1 antibody, pantothenate kinase 1 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
64 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 207 and 513 of PANK1 (Uniprot ID# Q8TE04)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed Hep G2, using PANK1(107-10571) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
107-10571 diluted at 1:1000
A: mouse liver
10% SDS PAGE
107-10571 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PANK1(107-10571) antibody at 1:500 dilution.
Sample(30 ug whole cell lysate)
A:Hep G2
7.5% SDS PAGE
107-10571 diluted at 1:5000
A:Hep G2
7.5% SDS PAGE
107-10571 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.