Anti-Parkin (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O60260
Gene Id	5071
Gene Symbols	PARK2
Protein Name / Synonyms	Parkin (C-term)
Clonality	Polyclonal
Preparation	This Parkin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-417 amino acids from the C-terminal region of human Parkin.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF, FC, IHC-P
Research Area	Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson disease identified to date. The function of Park2 is not well-known; however, it may play a role in the ubiquitin-mediated proteolytic pathway. Mutations in this gene are known to cause autosomal recessive juvenile parkinsonism. Alternative splicing of this gene produces three known products of undetermined function.

Specificity

Other Names

PARK2; PRKN; E3 ubiquitin-protein ligase parkin; Parkinson juvenile disease protein 2

NCBI Accession #

NP_004553.2;NP_054642.2;NP_054643.2

Other Accession #

NP_004553

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

51641

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; FC: 1:10-50; IHC: 1:50-100

	Park2 Antibody (C-term) (Cat# 102-16769) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Park2 antibody detected the Park2 protein (arrow).
	Confocal immunofluorescent analysis of Parkin Antibody (C-term)(Cat# 102-16769) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	Parkin Antibody (C-term) (Cat# 102-16769) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	The anti-Parkin (C-term) Pab (Cat# 102-16769) is used in Western blot to detect Parkin in mouse kidney tissue lysate.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human testis tissue reacted with PARK2 (Parkin) antibody (C-term) (Cat# 102-16769), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.