Rabbit Anti-Human Parkin (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O60260 |
| Gene Id | 5071 |
| Gene Symbols | PARK2 |
| Protein Name / Synonyms | Parkin (N-term) |
| Clonality | Polyclonal |
| Preparation | This Parkin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the N-terminal region of human Parkin. |
| Concentration (lot specific) | 0.410 mg/ml |
| Recommended Applications | Western Blotting, FC, IF, IHC-P |
| Research Area | Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson disease identified to date. The function of Park2 is not well-known; however, it may play a role in the ubiquitin-mediated proteolytic pathway. Mutations in this gene are known to cause autosomal recessive juvenile parkinsonism. Alternative splicing of this gene produces three known products of undetermined function.
Specificity
Other Names
PARK2; PRKN; E3 ubiquitin-protein ligase parkin; Parkinson juvenile disease protein 2
NCBI Accession #
NP_004553.2;NP_054642.2;NP_054643.2
Other Accession #
NP_004553
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
51641
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50; IHC: 1:10-50
 | Western blot analysis of lysate from SH-SY5Y cell line, using Park2 Antibody (N-term) (Cat# 102-16768). 102-16768 was diluted at 1:1000 at each lane. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. |
 | Parkin Antibody (N-term) (Cat# 102-16768) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of Parkin Antibody (N-term)(Cat# 102-16768) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
 | Parkin Antibody (N-term) (102-16768)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Parkin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.