Anti-PAX5 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q02548
Gene Id	5079
Gene Symbols	PAX5
Protein Name / Synonyms	PAX5 (Center)
Clonality	Polyclonal
Preparation	This PAX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the Central region of human PAX5.
Concentration (lot specific)	0.300 mg/ml
Recommended Applications	Western Blotting, FC, IHC-P
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.

Specificity

Predicted Reactivity

Mouse

Other Names

PAX5; Paired box protein Pax-5; B-cell-specific transcription factor

NCBI Accession #

NP_001267476.1;NP_001267477.1;NP_001267478.1;NP_001267479.1;NP_001267481.1;NP_001267482.1;NP_001267483.1;NP_001267484.1;NP_001267485.1;NP_057953.1

Other Accession #

Q02650; NP_057953.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

42149

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:10-50

	PAX5 Antibody (Center) (Cat# 102-11216) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PAX5 antibody detected the PAX5 protein (arrow).
	PAX5 Antibody (Center) (Cat# 102-11216) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	PAX5 Antibody (Center) (Cat# 102-11216)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PAX5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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