Anti-PAX6 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P26367
Gene Id	5080
Gene Symbols	PAX6
Protein Name / Synonyms	PAX6 (Center)
Clonality	Polyclonal
Preparation	This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the Central region of human PAX6.
Concentration (lot specific)	0.410 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Neuroscience
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Xenopus

Other Names

PAX6; AN2; Paired box protein Pax-6; Aniridia type II protein; Oculorhombin

NCBI Accession #

NP_000271.1;NP_001121084.1;NP_001245393.1;NP_001245394.1;NP_001595.2

Other Accession #

P55864; P63016; P63015; Q1LZF1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

46683

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	PAX6 Antibody (Center) (Cat# 102-17021) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the PAX6 antibody detected the PAX6 protein (arrow).
	Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of PAX6 Antibody (Center)(Cat# 102-17021) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
	Flow cytometric analysis of hela cells using PAX6 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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