Anti-PAX6-T373 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P26367
Gene Id	5080
Gene Symbols	PAX6
Protein Name / Synonyms	PAX6-T373
Clonality	Polyclonal
Preparation	This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-380 amino acids from human PAX6.
Concentration (lot specific)	0.250 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Neuroscience
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PAX6 encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly.

Specificity

Other Names

PAX6; AN2; Paired box protein Pax-6; Aniridia type II protein; Oculorhombin

NCBI Accession #

NP_000271.1;NP_001121084.1;NP_001245393.1;NP_001245394.1;NP_001595.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

46683

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	Western blot analysis of PAX6-T373 (Cat# 102-17022) in Y79 cell line lysates (35ug/lane). PAX6 (arrow) was detected using the purified Pab.
	Western blot analysis of PAX6 (arrow) using rabbit polyclonal PAX6 Antibody (T373) (Cat# 102-17022). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.
	PAX6-T373 Antibody (Cat# 102-17022) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PAX6-T373 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of PAX6-T373 Antibody(Cat# 102-17022) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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