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Anti-PBP2a Antibody, Biotinylated
Catalog #: 130-10307B-50
Mouse anti-PBP2a (5B9-G2-F12) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Staphylococcus spp |
| Host Species | Mouse |
| Accession Number | Q53707 |
| Gene Symbols | mecA |
| Protein Name / Synonyms | PBP2a |
| Clonality | Monoclonal |
| Clone | 5B9-G2-F12 |
| Immunogen | This antibody was produced by fusion of mouse myeloma with B cells obtained from a mouse immunized with a recombinant protein immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography. |
| Recommended Applications | ELISA (recommended dilution= 1:320, 000), Western Blotting (recommended dilution= 1:1, 000-5, 000), (see image) Western Blot: First Antibody: 1µ, g/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 12, 000X (HRP-Goat Anti-Mouse IgG) M: Protein Marker Lane 1: Recombinant PBP2a 1µ, g Lane 2: MSSA lysate 15µ, l |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Infectious Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
PBP2A has a low affinity for beta-lactam antibiotics such as methicillin and penicillin. This enables transpeptidase activity in the presence of beta-lactams, preventing them from inhibiting cell wall synthesis.
Specificity
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. The reconstituted antibody can be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C for many months without detectable loss of activity. Please avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.