Anti-PCK2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q16822
Gene Id	5106
Gene Symbols	PCK2
Protein Name / Synonyms	PCK2, PEPCK, PEPCK-M, PEPCK2, phosphoenolpyruvate carboxykinase 2, mitochondrial
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100
IP 1:20 - 1:100

Format

Calculated MW

47kDa/70kDa

Observed MW

71kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PCK2 (NP_004554.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PCK2 antibody (144-08446) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.
Western blot - PCK2 Polyclonal Antibody (144-08446)

Immunoprecipitation analysis of extracts of HepG2 cells using PCK2 antibody (144-08446). Western blot was performed from the immunoprecipitate using PCK2 antibody (144-08446) at a dilition of 1:1000.
Immunoprecipitation - PCK2 Polyclonal Antibody (144-08446)

Expiration:

12 months from the date of shipment when stored properly.

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