Anti-PCM-1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q15154
Gene Id	5108
Gene Symbols	PCM1
Protein Name / Synonyms	PCM-1 (Center)
Clonality	Polyclonal
Preparation	This PCM-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 632-661 amino acids from the Central region of human PCM-1.
Concentration (lot specific)	0.250 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.

Specificity

Other Names

PCM1; Pericentriolar material 1 protein

NCBI Accession #

NP_006188.3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

228533

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Fluorescent image of A549 cell stained with PCM-1 Antibody (Center)(Cat# 102-17381/SA120711BC).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.
	Western blot analysis of PCM-1 antibody (Center)(Cat# 102-17381) in Ramos cell line lysates (35ug/lane). PCM-1 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PCM-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	PCM-1 Antibody (Center) (Cat# 102-17381) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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