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Anti-PCNA Antibody
Catalog #: 130-10040
Rabbit Anti-Human PCNA Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | The antibody can specifically bind to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. The specificity for binding to recombinant protein, cellular protein and native antigen is not defined. Cross reactivity with mouse and rat PCNA has not yet been tested. |
| Accession Number | P12004 |
| Gene Id | 5111 |
| Gene Symbols | PCNA |
| Clonality | Polyclonal |
| Immunogen | Immunogen was recombinant protein derived from human PCNA. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation, and followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:2000 (at least detecting 500ng/ml)) |
| Antigen Catalog Number | 230-00030 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can also be stored over months at 4°C. Please avoid freeze-thaw cycles. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Proliferating cell nuclear antigen acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Expression of PCNA or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerasedelta to play a fundamental role in the initiation of cell proliferation.
Expression of PCNA or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerasedelta to play a fundamental role in the initiation of cell proliferation.
Typical Data
Storage/Stability
Keep it at 4°C if intended for use within a month. Store at -20°C if over months. Minimize freezing and thawing when use.
Expiration
12 months from the date of shipment when stored properly.
References
- Matunis MJ.(2002) On the road to repair: PCNA encounters SUMO and ubiquitin modifications. Mol Cell. 10(3):441-2.
- Uberti D, et al..(2003) Involvement of DNA damage and repair systems in neurodegenerative process. Toxicol Lett. 4;139(2-3):99-105.