Rabbit Anti-Human PCNA (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P12004 |
| Gene Id | 5111 |
| Gene Symbols | PCNA |
| Protein Name / Synonyms | PCNA (C-term) |
| Clonality | Polyclonal |
| Preparation | This PCNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-261 amino acids from the C-terminal region of human PCNA. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
Specificity
Predicted Reactivity
Rat, Bovine, Hamster, Monkey
Other Names
PCNA; Proliferating cell nuclear antigen; Cyclin
NCBI Accession #
NP_002583.1;NP_872590.1
Other Accession #
P04961; P61258; P57761; Q3ZBW4
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
28769
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
 | Western blot analysis of PCNA Antibody (C-term) (Cat# 102-15172) in Jurkat, Hela, 293 cell line lysates (35ug/lane). PCNA (arrow) was detected using the purified Pab. |
 | Western blot analysis of PCNA (arrow) using rabbit polyclonal PCNA Antibody (C-term) (Cat# 102-15172). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PCNA gene. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PCNA antibody (C-term) (Cat# 102-15172), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of PCNA Antibody (C-term) (Cat# 102-15172) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
 | Confocal immunofluorescent analysis of PCNA Antibody (C-term) (Cat# 102-15172) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue). |
 | PCNA Antibody (C-term) (Cat# 102-15172)immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCNA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
