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Anti-PDCD6IP Antibody
Catalog #: 144-02215
PDCD6IP Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q8WUM4 |
| Gene Id | 10015 |
| Gene Symbols | PDCD6IP |
| Protein Name / Synonyms | PDCD6IP, AIP1, ALIX, DRIP4, HP95, programmed cell death 6-interacting protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.09% Sodium azide, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
30kDa/96kDa
Observed MW
109kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PDCD6IP (NP_037506.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using PDCD6IP antibody (144-02215) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using PDCD6IP antibody (144-02215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using PDCD6IP antibody (144-02215) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PDCD6IP antibody (144-02215) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.