Anti-PDE1B Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q01064
Gene Id	5153
Gene Symbols	PDE1B
Protein Name / Synonyms	PDE1B, HEL-S-79p, PDE1B1, PDES1B, phosphodiesterase 1B
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

59kDa/61kDa

Observed MW

59kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-536 of human PDE1B (NP_000915.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PDE1B antibody (144-02102) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - PDE1B antibody (144-02102)

Immunohistochemistry of paraffin-embedded rat brain using PDE1B antibody (144-02102) at dilution of 1:100 (40x lens).
Immunohistochemistry - PDE1B antibody (144-02102)

Immunofluorescence analysis of U2OS cells using PDE1B antibody (144-02102). Blue: DAPI for nuclear staining.
Immunofluorescence - PDE1B antibody (144-02102)

Expiration:

12 months from the date of shipment when stored properly.

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