Anti-PDGFRA (C-term E1063) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P16234
Gene Id	5156
Gene Symbols	PDGFRA
Protein Name / Synonyms	PDGFRA (C-term E1063)
Clonality	Polyclonal
Preparation	This PDGFRA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1048-1077 amino acids from the C-terminal region of human PDGFRA.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

Specificity

Predicted Reactivity

Rat, Chicken

Other Names

PDGFRA; PDGFR2; RHEPDGFRA; Platelet-derived growth factor receptor alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2; CD_antigen=CD140a; Flags: Precursor

NCBI Accession #

NP_006197.1

Other Accession #

P20786; P26618; Q9PUF6

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

122670

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of PDGFRA Antibody (C-term E1063) (Cat# 102-17532) in K562 cell line lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.
	Western blot analysis of PDGFRA antibody (C-term E1063) (Cat# 102-17532) in mouse lung tissue lysates (35ug/lane). PDGFRA (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	PDGFRA Antibody (C-term E1063) (Cat# 102-17532) flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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