Rabbit Anti-PDK2 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat, Non-Human Primate (rhesus monkey) |
| Host Species | Rabbit |
| Accession Number | Q15119 |
| Gene Id | 5164 |
| Gene Symbols | PDK2 |
| Protein Name / Synonyms | PDK2 (C-term) |
| Clonality | Polyclonal |
| Preparation | This PDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-407 amino acids from the C-terminal region of human PDK2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Specificity
Other Names
PDK2; PDHK2; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial; Pyruvate dehydrogenase kinase isoform 2
NCBI Accession #
NP_001186827.1;NP_001186828.1;NP_002602.2
Other Accession #
Q64536; Q9JK42
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
46154
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of PDK2 Antibody (C-term)(Cat# 102-17090) in mouse intestine tissue lysate (35ug/lane). PDK2 (arrow) was detected using the purified Pab. |
 | Western blot analysis of PDK2 (arrow) using PDK2 Antibody (C-term)(Cat# 102-17090). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDK2 gene (Lane 2) (Origene Technologies). |
 | PDK2 Antibody (C392) (Cat# 102-17090) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PDK2 antibody detected the PDK2 protein (arrow). |
 | Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.