Anti-PDX1 (T11) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O00330
Gene Id	8050
Gene Symbols	PDHX
Protein Name / Synonyms	PDX1 (T11)
Clonality	Polyclonal
Preparation	This PDX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human PDX1.
Concentration (lot specific)	0.350 mg/ml
Recommended Applications	IF, Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PDX1, located in the mitochondrial matrix, is required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by noncovalent bonds. Defects in PDHX are a cause of lacticacidemia. PDX1 belongs to the 2-oxoacid dehydrogenase family and contains 1 lipoyl-binding domain.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

PDHX; PDX1; Pyruvate dehydrogenase protein X component, mitochondrial; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; E3-binding protein; Lipoyl-containing pyruvate dehydrogenase complex component X; proX

NCBI Accession #

NP_001128496.1;NP_001159630.1;NP_003468.2

Other Accession #

P52947; P52946; P52945

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

54122

Recommended Dilutions

IF: 1:100; WB: 1:1000; IHC: 1:10-50

	Fluorescent confocal image of SY5Y cells stained with PDX1 (T11) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-14684 PDX1 (T11) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). Note the highly specific localization of the PDX1 immunosignal to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000110435).
	Western blot analysis of anti-PDX1 Antibody (T11) (Cat# 102-14684) in NCI-H460 cell line lysates (35ug/lane).PDX1-T11(arrow) was detected using the purified Pab.
	Western blot analysis of PDX1 (arrow) using PDX1 Antibody (T11) (Cat# 102-14684). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-PDX1-T11.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products