Phospho-PEA15-S104 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15121 |
| Gene Id | 8682 |
| Gene Symbols | PEA15 |
| Protein Name / Synonyms | PEA15, HMAT1, HUMMAT1H, MAT1, MAT1H, PEA-15, PED, astrocytic phosphoprotein PEA-15 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
15kDa/17kDa
Observed MW
13kDa
Immunogen
A synthetic phosphorylated peptide around S104 of human PEA15 (NP_003759.1).
Modification
Phospho S104
Storage
-20°C
Images
Western blot analysis of extracts of HeLa and C6 cells, using Phospho-PEA15-S104 antibody (144-61521) at 1:2000 dilution. HeLa cells were treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.C6 cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.
Expiration:
12 months from the date of shipment when stored properly.
