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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 5226 |
| Protein Name / Synonyms | PGD |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
6PGD antibody, PGD antibody, "6-phosphogluconate dehydrogenase, decarboxylating antibody", phosphogluconate dehydrogenase antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
53 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 201 of PGD (Uniprot ID# P52209)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PGD protein stained by PGD antibody [N1N3] (107-10279) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PGD protein stained by PGD antibody [N1N3] (107-10279) diluted at 1:500.
Blue: Hoechst 33342 staining.
PGD antibody [N1N3] detects PGD protein at cytoplasm on A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
PGD antibody [N1N3] (107-10279) dilution: 1:500.
Sample: Paraffin-embedded A549 xenograft.
PGD antibody [N1N3] (107-10279) dilution: 1:500.
Sample(30 ug whole cell lysate)
A:293T
B:MOLT4
C:Raji
10% SDS PAGE
107-10279 diluted at 1:1000
A:293T
B:MOLT4
C:Raji
10% SDS PAGE
107-10279 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.