Anti-PGK1 (Center S320) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P00558
Gene Id	5230
Gene Symbols	PGK1
Protein Name / Synonyms	PGK1 (Center S320)
Clonality	Polyclonal
Preparation	This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-334 amino acids from the Central region of human PGK1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Also known as ATP:3-phosphoglycerate 1-phosphotransferase, this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. Phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and is proposed to participate in the angiogenic process as a disulfide reductase. Mutations in PGK1 may be associated with hemolytic anemia.

Specificity

Predicted Reactivity

Monkey

Other Names

PGK1; PGKA; Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2

NCBI Accession #

NP_000282.1

Other Accession #

Q60HD8; Q5J7W1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

44615

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of lysates from A431, Hela, U-937, BA/F3 cell line (from left to right), using PGK1 Antibody (S320) (Cat# 102-17157). 102-17157 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 Antibody (Center S320)(Cat# 102-17157), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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