Anti-PHB2 (Y248) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q99623
Gene Id	11331
Gene Symbols	PHB2
Protein Name / Synonyms	PHB2 (Y248)
Clonality	Polyclonal
Preparation	This PHB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-255 amino acids from human PHB2.
Concentration (lot specific)	0.440 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

PHB2 acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. It functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity and is probably involved in regulating mitochondrial respiration activity and in aging.

Specificity

Predicted Reactivity

Rat, Bovine

Other Names

PHB2; BAP; REA; Prohibitin-2; B-cell receptor-associated protein BAP37; D-prohibitin; Repressor of estrogen receptor activity

NCBI Accession #

NP_001138303.1;NP_001254629.1

Other Accession #

Q5XIH7; O35129; Q2HJ97; NP_009204

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

33296

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of PHB2-Y248(Cat# 102-17234) in MDA-MB435 cell line lysates (35ug/lane).PHB2 (arrow) was detected using the purified Pab.
	Western blot analysis of PHB2-Y248 (Cat# 102-17234) in mouse kidney tissue lysates (35ug/lane). PHB2 (arrow) was detected using the purified Pab.
	Western blot analysis of PHB2 (arrow) using PHB2 Antibody (Y248) (Cat# 102-17234). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies).
	PHB2 Antibody (Y248)(Cat# 102-17234) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHB2 Antibody (Y248) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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