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Anti-PHGDH (Center) Antibody
Catalog #: 102-15242
Rabbit Anti-Human PHGDH (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O43175 |
| Gene Id | 26227 |
| Gene Symbols | PHGDH |
| Protein Name / Synonyms | PHGDH (Center) |
| Clonality | Polyclonal |
| Preparation | This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human PHGDH. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.
Specificity
Predicted Reactivity
Mouse, Rat, Monkey
Other Names
PHGDH; PGDH3; D-3-phosphoglycerate dehydrogenase
NCBI Accession #
NP_006614.2
Other Accession #
O08651; Q61753; Q60HD7; NP_006614
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
56651
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | PHGDH Antibody (Center) (Cat# 102-15242) western blot analysis in Ramos cell line lysates (15ug/lane).This demonstrates the PHGDH antibody detected the PHGDH protein (arrow). |
 | PHGDH Antibody (Center) (Cat# 102-15242) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHGDH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | PHGDH Antibody (Center) (Cat# 102-15242) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.