Anti-PHKA2 (C-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	5256
Protein Name / Synonyms	PHKA2, C-term
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

GSD9A antibody, MGC133071 antibody, PHK antibody, PYK antibody, PYKL antibody, XLG antibody, XLG2 antibody, PHKA2 antibody, phosphorylase kinase alpha-subunit antibody, "phosphorylase b kinase regulatory subunit alpha, liver isoform antibody", phosphorylase b kinase regulatory subunit alpha liver isoform antibody, phosphorylase kinase alpha L subunit antibody, "phosphorylase kinase, alpha 2 (liver) antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

138 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 1172 and 1235 of PHKA2 (Uniprot ID# P46019)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Colon ca, using PHKA2(107-10757) antibody at 1:250 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
5% SDS PAGE
107-10757 diluted at 1:500

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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